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Rheum species is a perennial stout herb, distributed in the temperate and sub tropical regions of Himalaya from Kashmir to Sikkim . The genus Rheum have been domesticated as medicinal plants for human consumption as its roots and stem are rich in anthraquinones such as Emodin and Rhein.Standardization of protocol for the micro propagation of Rheum using different types of explants and culture media supplemented with various types of growth hormones was carried out. Different parts (seeds, leaves, shoots and rhizomes) of Rheum were used as explants for tissue culture. These explants were first washed in laboline and tween 20 and after surface sterilization using different chemical sterilents, were cultured on different media and in combinations of phytohormones. All the explants showed good germination response with whole plant and roots exhibited about (98%) response each while as seeds exhibited only 95% germination.

In vitro micro propagation of Rheum explants supplemented with various types of growth hormones