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The present research work was undertaken to choose the most effective sugar concentration in in vitro microtuberization from cultured shoot tips and to select the best substrates for minituber production from ex vitro transferred plantlets. Percentage of survivability of microtuber producing plants increased with the increase of sucrose concentration. Best survival rate and average diameter of tuber per plant was found at 10% sucrose. Minituber production on direct field showed best performance. It was observed that after 30 days, maximum shoot length per plant (188.87 mm) was found on the direct field whereas the lowest shoot length per plant (66.81 mm) was on coconut dust. Lowest weight of tuber per plant was also recorded on the substrates containing coconut dust. The highest weight of tuber was found in direct field. In case of, average diameter of tuber per plant, 25% soil + 75% coconut dust showed minimum result and on the other hand, in direct field, large size of tubers were found.

Effect of different levels of sucrose on microtuberization and different substrates on minituber production resulted from potato meristem culture