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Alkaline agar gel electrophoresis is a basic manual laborious technique for identifying Hemoglobin variants in the screening for Hemoglobinopathies. It’s a cheaper alternative to the now preferred method of detection of Hb variants, i.e., HPLC. In this study, at a government hospital clinical biochemistry laboratory setup, we try to compare and emphasize the importance of this manual procedure. We have done this observation from the archives of lab and case sheet data for the past four and half years; that 1891 electrophoresis were done and 65% were normal, 20% shown Thalassemia trait, and 6% were Thalassemia major and 8% were shown sickle Hb pattern. Majority correlated clinically with the electrophoresis report and in doubtful cases (1.32%), HPLC confirmed the electrophoresis report. Hence wherever feasible, we recommend this manual alkaline agar gel electrophoresis where cost is a limiting factor.

Agar Gel Alkaline Electrophoresis As A Cost Effective Screening Modality For Abnormal Hemoglobins