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Background: Antimicrobial activity of root canal materials may help to eliminate residual microorganisms unaffected by the effects of both chemo-mechanical preparation and intracanal medication. Mineral trioxide aggregate (MTA), is one such bioactive material that has potential antimicrobial property. The principle aim of this study was to evaluate the antibacterial properties of ProRoot WMTA against E. faecalis and to compare the antibacterial effect of WMTA when mixed with 2% Chlorhexidine (CHX), and Phosphate Buffered Saline (PBS), as a substitute for sterile water against Enterococcus faecalis (E. faecalis). Method: ProRoot WMTA was divided into three groups one for each hydrating agent. The antimicrobial effect was determined by placing freshly mixed MTA samples in wells created in trypticase soy agar (TSA) plates inoculated with Enterococcus faecalis. These plates were maintained at room temperature for 2 hours for prediffusion of the materials and incubated at 37 0 C for 24 hours and zones of inhibitions were recorded. Results: The samples of ProRoot WMTA irrespective of the mixing agent showed antimicrobial activity against E. faecalis. Higher mean zone of inhibition was shown by WMTA/ CHX followed by WMTA/PBS and WMTA/Sterile Water. The difference between the three groups were found to be statistically significant with P value <0.001. Conclusion: Phosphate buffered saline (PBS) and 2% Chlorhexidine enhanced the antibacterial efficacy to inhibit E. faecalis and can be recommended to be used as mixing agent for WMTA as substitute to sterile water provided by manufacturer.

iosr journal of dental and medical sciences

Effect of Two Different Hydrating Solutions on Antibacterial Property of White Mineral Trioxide Aggregate Against Enterococcus Faecalis - An in Vitrocomparative Study