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Aim:The aim of the present study was to evaluate the antimicrobial activity of different root canal sealers (MTA Fillapex, Real Seal SE, Acroseal and Zinc Oxide Eugenol) against Enterococcus fecalis and Candida albicans. Materials and method : The antimicrobial effect of different root canal sealers was evaluated by the direct contact with agar diffusion method. The freshly prepared mixed sealers were placed in prepared wells of agar plates inoculated with E. faecalis and C.albicans separately. All plates were incubated for 7 days at 37°C under aerobic conditions, and zones of inhibition were measured at 24, 48, 72 hours and 7 days. All statistical analyses were performed with the SPSS (PC version 10 software, IBM, NY, USA) statistical software. Result : The sealer MTA fillapex showed highest antimicrobial activity against C. albicans (16.0 ± 0.20 mm) followed by Real Seal SE (15.2 ± 0.20 mm). Amongst the tested sealers only MTA Fillapex showed antimicrobial activity against E. faecalis (12.40± 0.20 mm). No antimicrobial activity was observed with zinc oxide eugenol and Acroseal. Conclusion : Amongst the tested sealers MTA Fillapex and Real Seal SE both showed antimicrobial activity against C.albicans whereas only MTA Fillapex showed antibacterial activity against E. faecalis. Acroseal and Zinc Oxide Eugenol did not show any antimicrobial activity.

Antimicrobial Activity of MTA Fillapex, Real Seal SE, Acroseal and Zinc oxide Eugenol Sealers against Enterococcus Faecalis and Candida Albicans