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Klebsiella pneumoniae has been associated with different types of infections and one of the most important aspects of Klebsiella is the emergence of multi drug resistant strains particularly those involved in nosocomial diseases. Materials and methods: A total number of 800 sputum, 148 pus and 850 urine samples from September 2013 to August 2014 were included in the study. Isolates of Klebsiella pneumoniae were identified by their standard microbiological techniques and were subjected to antibiotic sensitivity testing by modified Kirby-Bauer disc diffusion method. Results: Of the 1798 samples processed 410 (22%) samples were culture positive for various organisms. A total of 410 organisms were isolated, of them 157 (38%) were Klebsiella pneumoniae. The frequency of ESBL producers in our study was 70 (44%) of all Klebsiella isolates. Conclusion: The present study reveals 8.73% of isolation rate of Klebsiella pneumoniae from various clinical samples with highest sensitivity to amikacin and highest resistance to cefotaxime and ceftriaxone. Prevalence of ESBLs among members of Enterobacteriaceae constitutes a serious threat to the current beta-lactam therapy,leading to treatment failure. There is an urgent need to emphasize rational use of drugs to minimize the misuse of available antimicrobials.

iosr journal of dental and medical sciences

Isolation And Antibiotic Susceptibility Pattern With Esbl Production Of Klebsiella Pneumoniae Isolated From Sputum, Pus And Urine Samples In A Tertiary Care Hospital.