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Non-availability of CLSI guidelines and presence of some degree of confusion to clinician in regard to intermediate susceptibility, have led to refer to EUCAST guidelines. The aim of this study was to compare the CLSI and EUCAST inhibitory zone diameters by disk diffusion. Total 100 clinical isolates of Enterobacteriaceae were analysed. Inhibition zone diameters were determined for Cefotaxime, Ceftazidime, Piperacillin Tazobactam and Nitrofurantoin. CLSI and EUCAST guidelines were applied .For Escherichia coli, the resistance rate to cefotaxime as per CLSI and EUCAST was 100% for each, for Ceftazidime 100% for each, for Piperacillin Tazobactam 77.4% for each. For Klebsiella ,resistance rate to cefotaxime as per CLSI and EUCAST was 94.3% and 62.8%,for Ceftazidime 100% for each, for Piperacillin Tazobactam 48.6% and 68.6%.For Proteus,resistance rate to cefotaxime as per CLSI and EUCAST to was 100% for each,for Ceftazidime 100% for each, for Piperacillin Tazobactam 71.4 % and 100%.For Pseudomonas,resistance rate to ceftazidime as per CLSI and EUCAST was 59.3% and 66.7%,for Piperacillin Tazobactam 26% and 40.7%.The resistance rate to Nitrofurantoin as per CLSI and EUCAST for Escherichia coli was 10% and 10%, Klebsiella 0% and 28.6%,Pseudomonas 100% and 100%.We conclude from the observations of our study that antibiotic susceptibility testing by EUCAST guidelines is more feasible than CLSI guidelines to both the microbiologist and clinician.

Comparision of Antibiotic Susceptibility Testing As Per CLSI and Eucast Guidelines for Gram Negative Bacilli