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This study compares t wo bacterial expression (Prevotel la intermedia , Prevote l la nigresens) and their expression in p regnant and gestat ional diabet ic pat ients wi th periodontal disease and proposes to explore the microbiologic n ature of the associat ion between early colonizers, such as P.intermedia and P.nigresens are bound by later colonizers such as P. gingival is and T. forsythia . Materials and methods: Forty pregnant women in each group wi th Chronic Periodont i t is wi th and wi thout gestat ional diabetes, were grouped into two groups – as a case control study. Plaque samples from these pat ients were col lected using paper points. The samples were evaluated using Real Time –Polymerase Chain Reaction technique. Resul ts: This study resul ted in an increased presence of early colonizers in both groups , showing a “3 fold increase” of Prevotel la intermedia among pregnant pat ients wi t h diabetes and periodontal disease and showed a “1 fold increase” among pregnant pat ient pat ients wi thout diabetes compared to Prevotella nigrescens. Conclusion: The current study indicates that Prevotel la intermedia is signi f icant ly increased among the gestat ional diabet ic and therefore involved in the demonstrated microbial shi f t during pregnancy and Gestational Diabetes Meli tus and more prone for periodontal breakdown.

Comparative expression of periodontal pathogens in pregnant women with periodontitis and diabetes in Indian population – A case-control study