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Purpose: To evaluate the performance of the Combination Double Disc Method in the identification of ESBL-producing Enterobacteriaceae isolates. Materials and Methods: A total of 44 clinically relevant Enterobacteriaceae isolates were examined. The ESBL classification furnished by Combination Double Disc Test Method was concordant with that of the comparison method (Etest ESBL and molecular identification of beta-lactamase genes) for 18 (41%) of the 44 isolates evaluated. Results: ESBL production was correctly detected in 41% ESBL-producing organisms with at least one of the combination disks (sensitivity, 80%). Conclusion: In our study, Combination Double Disc Test Method appears to be a rapid and reliable tool for routine identification of ESBL-producing isolates of Enterobacteriaceae.

iosr journal of dental and medical sciences

Detection of extended-spectrum ß-lactamases in members of the family Enterobacteriaceae: comparison of the Combination Double Disc Test Method and the Etest ESBL.