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Sex determination is not only a useful tool but of extreme necessity in forensic investigations specifically in cases of sexual assault, aged blood stains and human skeletal remains whereby rapid, sensitive, accurate and reliable methods of investigation are of the utmost importance. A forensic study for genetic sex determination of burnt powdered skeletal fragments from Sokoto, Northwestern Nigeria, using amelogenin gene and alphoid repeats primers was undertaken. With amelogenin gene primers, a success rate of 33.3% was recorded for correct genetic sex identification. PCR Sensitivity = 100%, Specificity = 25%, Predictive value of positive test = 40%, Predictive value of negative test = 100%, False positive rate = 300%, False negative rate = 0%, Efficiency of test = 50%. Fisher's exact probability test P = 1. Z-test: z-value = 0.7746, p>0.05; not statistically significant. Alphoid repeats primers, yielded a success rate of 50% for correct genetic sex identification. PCR Sensitivity = 100%, Specificity = 33%, Predictive value of positive test = 60%, Predictive value of negative test = 100%, False positive rate = 200%, False negative rate = 0%, Efficiency of test = 83%. Fisher's exact probability test P = 1. Z-test: z-value = -2.02837, p<0.05; statistically significant. This study has demonstrated the use of amelogenin gene and alphoid repeats in forensic study for genetic sex determination of burnt powdered skeletal fragments from Sokoto, Northwestern Nigeria, and that alphoid repeats yielded better results than amelogenin gene. This is the first known study determining the sex of burnt cadaveric skeletal fragments by means of PCR in Nigeria. There is need for further studies in Nigeria to complement the findings of this study.

Forensic Study for Genetic Sex Determination of Burnt Powdered Skeletal Fragments from Sokoto, Northwestern Nigeria.