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Syngas fermentation by acetogenic bacteria present a major bottleneck, which is the low solubility of the substrates (CO and CO2) and, therefore, the low productivity caused by low cell density. In lab scale, many studies are conducted in serum bottles and agitated in incubation shakers with bottles kept vertically. In the present study, the serum bottles were kept horizontally in a new strategy to increase turbulence and, as a consequence, the mas transfer. Cell growth of Clostridium carboxidivorans (DSM 15243), Clostridium ragsdalei (DSM 15248) and Clostridium ljungdahlii (DSM 13528) was increased by the increase in the availability of CO and CO2 in the liquid medium. Cell growth of C.carboxidivoransincreased 3.7 times and the specific growth rate became 50% higher. Ethanol and acetic acid production were also favored by this strategy. C.carboxidivorans was thebest specie for ethanol production.

A New Strategy for Acetogenic Bacteriacell Growth and Metabolites Production Using Syngas in Lab Scale