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Chlorophytum borivilianum belonging to the family Liliaceae is commonly known as safed musli. It is a perennial rhizomatous herbs widely distributed in pan tropical region which containing pharmaceutical important compound saponins. Safed musli is an endangered medicinal herb value for its dried roots possesses immune-modulatory and aphrodisiac properties that form an important ingredient of herbal tonic. Root culture, callus established from micro propagated plants of C.borivilianum on MS medium supplemented with 1 mg/l IBA+10% CM & 0.5 mg/l 2, 4 + 15% CM and Leaf base & Root are explants. Growth kinetics of explants done under portable incubation chamber where natural ambient light, temperature and relatively humidity are controlled. Maximum biomass observed after 39 days of inoculation after sub culturing of these culture root growth shown and even number of roots increased from one to three. Resulted cost reduction on electricity & synthetic hormones in micro propagation compared to the generally used PTC technology & large scale of carbon emission in the atmosphere and polluted to the environment can be controlled by use of this protocol.

Cost Effective Protocol for In Vitro Culture of Chlorophytum borivilianum Sant. Et Fernand.