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Objective: To assess the knowledge and practice of workers, professionals and managers about the Healthcare Waste in a reference institute in tropical and communicable diseases Teresina (PI). Method: A descriptive, observational study with quantitative analysis, conducted from 01 to 30 March 2012. The study population was composed of: 49 workers, 124 health workers and 11 managers sector. Data were collected from interviews, using three instruments with a specific script to each group's structured. The study was approved by the ethics committee on research, CEP-UFPI with CAAE: 0210.0.045.000-11. Results: 90% of workers, 95,2% of professional and 54,5% of the managers demonstrated ignorance of the existence of the Plan for Waste Management Health Service (PGRSS) of the institution. Conclusion: There was a deficit in the knowledge of workers, professionals and managers about Plan for Waste Management Health Service and improper practices in the management of Healthcare Waste

Knowledge and practice of workers, professionals and managers on waste of health services