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An indirect ELISA test was developed and standardized for the diagnosis of caseous lymphadenitis in goats. The culture supernatant of a 48h culture of Corynebacterium pseudotuberculosis cultivated in BHI broth was used as antigen. In order to determine the cut off and the evaluation of the sensibility and the specificity, thirty one serum samples from infected goats with caseous lesions, whose presence of C. pseudotuberculosis was confirmed by positive culture, were used, as well as fifty six serum samples from no infected goats. The sensitivity and the specificity of the test were calculated by ROC analysis. The sensitivity and the specificity of the ELISA BHI test were 93.5% and 100%, respectively.

Determinação da sensibilidade e da especificidade de um teste de ELISA indireto para o diagnóstico de linfadenite caseosa em caprinos.