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This investigation deals with the effect of paper mill effluent, on the histochemical components of the liver of Rasbora daniconius . The Paper mill effluent was collected directly from the 45 Km away from Aurangabad (MS) India. Histochemical studies on protein, lipid and glycogen contents of liver Rasbora daniconius showed a progressive decrease in staining intensity to Mercury bromophenol blue (Hg-BPB), Sudan black B and Bets’s Carmine at 30 days exposure when treated at sublethal concentrations (1.9% and 0.95%) of paper mill effluent. The magnitudes of these changes were dose dependent. This study, therefore, concludes that paper mill effluent creates metabolic crisis and impairment in fish liver. The depletion in level of protein, lipid and glycogen points towards exhaustion of cell- energy to meet high demand of fish in stressful condition.

Histochemical changes in the liver of freshwater fish, Rasbora daniconius, exposed to paper mill effluent