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A comparative bio-molecular assay was made on the two cyanobacterial isolates i.e. Anabaena cylindrica and Oscillatoria tenue to study the effect of different concentration of Diazinon, an organophosphorus insecticide. We have carried out these experiments to investigate the response of different concentration of Diazinon. Finally Maximum Allowable Concentration (MAC) of Diazinon on Cyanobacterial isolates was observed. Heterocystous forms of cyanobacteria are more sensitive to pesticide than that the non-heterocystus forms of cyanobacteria. Cyanobacterial isolates were treated with different concentrations of Diazinon i.e., 1ppm, 3ppm, 5ppm, 7ppm & 10ppm. The growth, macromolecular content of carbohydrate, protein, amino acid and nitrogen was observed. The experiments were carried out for 28 days, and it was observed that the higher concentration i.e., 9ppm and 10ppm of Diazinon are adversely affecting the growth & the macromolecular contents of cyanobacterial isolates. It is also found that at late exponential phase (even in untreated conditions) the experimental macromolecular content was high in the isolates.

Bio-Molecular assay of cyanobacteria on response to diazinon an Organophosphorus insecticide.