Open AccessMOLECULAR PHYLOGENETIC STUDY OF LUFFA TUBEROSA ROXB. (CUCURBITACEAE) BASED ON INTERNAL TRANSCRIBED SPACER (ITS) SEQUENCES OF NUCLEAR RIBOSOMAL DNA AND ITS SYSTEMATIC IMPLICATION
Author(s) -
Ajmal Ali M,
S. Karuppusamy,
Mohd Fahad,
Al Hemaid
Publication year - 2010
Publication title -
international journal of bioinformatics research
Language(s) - English
Resource type - Journals
eISSN - 0975-9115
pISSN - 0975-3087
DOI - 10.9735/0975-3087.2.2.42-60
Subject(s) - momordica , biology , cucurbitaceae , internal transcribed spacer , ribosomal dna , phylogenetic tree , botany , genus , trichosanthes , traditional medicine , genetics , gene , medicine , alternative medicine , traditional chinese medicine , pathology
The phylogenetic position of long been debatable species Luffa tuberosa was inferred in the present study using ITS sequence of nuclear ribosomal DNA data. The study sampled a total number of 16 accessions which include five accessions of Luffa (under four species i.e. Luffa acutangula, L. cylindrica, L. aegyptiaca and L. tuberosa ), nine accessions of Momordica (under eight species i.e. M. angustisepala , M. balsamina , M. cabraei , M. charantia , M. charantia subsp. macroloba , M. cissoides, M. cochinchinensis , M. dioica and M. foetida ) and two accessions of Trichosanthes under two species (i.e. T. lepiniana and T. tricuspidata ). The sequence data analysis clearly reveals nesting of Luffa tuberosa within the clade of Momordica , thus, we herein support the inclusion of Luffa tuberosa into the genus Momordica as M. tuberosa (Roxb.) Cogn.
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