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Procuring considerable quantity of DNA of nearly fair quality from trees is difficult because of the presence of metabolites and mucilaginous substances, which is found in excess in their leaves that interfere with the isolation procedures. Gmelina arborea, a fast growing species of the family verbenaceae occurs naturally in tropical and sub tropical regions of Asia. Currently, though there are a number of methods for isolation of DNA like the CTAB method, SDS Method etc., no single method was suitable for obtaining fine quality DNA from the leaves of Gmelina arborea for further molecular studies because of the presence of polyphenols and mucilaginous substances in the leaves. Herein we describe a modified protocol based on Dellaporta et al. (1983) for isolating DNA from dried leaves of Gmelina arborea, which is suitable for further molecular analysis. The protocol overcomes the problem of coprecipitation with contaminating agents. The yield of the DNA was found to be 50μg from 0.2 g of starting material. The purity of the DNA was also checked by the absorbance ratios at 260nm and 280nm respectively. The DNA thus obtained was used for RAPD and ISSR analysis. KeywordsDNA extraction, dried leaves, Gmelina arborea, molecular analysis

A rapid method for isolation of high quality DNA from leaves of Gmelina arborea (Roxb) for molecular analysis