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Aims: The objective was to compare the sedimentation spontaneous in tube technique (SSTT), the direct smear, the zinc sulfhate (surface film and sediment analyzed) methods with the formalin-ethyl acetate method as the reference standard. Study Design: This a cross-sectional study performed in two populations around Chapala lake, Jalisco, Mexico. Place and Duration of Study: Sample Department of Medical Sciences and Life, University Center of the Ciénega, University of Guadalajara, Ocotlán, Jalisco, Mexico. Original Research Article Madriz-Elisondo et al.; IJTDH, 41(4): 52-63, 2020; Article no.IJTDH.56558 53 Department of Physiology, Health Sciences University Center. University of Guadalajara, Jalisco. México, between June 2018 and July 2019. Methodology: Sample: We included a total of 297 samples and were analyzed by the direct smear, the zinc sulfhate (surface film and sediment analyzed), the SSTT and formalin-ethyl acetate methods. Results: The SSTT was able to detect 40% of intestinal parasites, very good agreement for Entamoeba complex, Entamoeba coli, and Ascaris lumbricoides kappa index=0.697, 0.791, 0.696, respectively and excellent agreement for Giardia lamblia kappa index = 0.843. Regarding the isolation of Blastocystis spp, only a poor agreement was found among all techniques. The SSTT was able to detect multiple parasites with a sensitivity of 82.90% and specificity 83.80 kappa index= 0.649. Conclusion: The SSTT showed a very good agreement for the diagnosis intestinal polyparasitism which could represent another alternative for the concentration and identification protozoans and intestinal helminthes in low-resource settings.

international journal of tropical disease and health

Comparison of Three Parasitological Stool Examination Methods with the Formalin-Ethyl Acetate Procedure for the Diagnosis of Intestinal Parasites in Humans