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The aim of this study was to correlate analytical methods (HPLC and spectrophotometric) in assessing the changes in total β-carotene contents in leafy vegetables during ambient temperature storage (29+2°C) and domestic processing (5 min,100°C.) The vegetables analyzed were: Telfairia occidentalis, Amaranethus hybridus, Talinum triangulare,Pterocarpus mildbraedli and Gnetum africanum. Total–carotene was determined spectrophometrically, while HPLC was used for detailed analysis of carotenoides. Lutein, β -cryptoxanthin and β-carotene isomers were identified and quantified. Results indicated that the raw vegetables were rich in lutein (124.03-655.95 μg/gdwt) and total β -carotene (45.42 – 246.93 μg/gdwt). Beta–cryptoxanthin was detected in small quantity (5.05-11 μg/gdwt). However, spectrophotometric result indicated a total–carotene content range (186.10 – 953.78 μg/gdwt). Cooking increased significantly (P< 0.05), the lutein (382. 92 – 1158.83 μg/gdwt), total β -carotene (738.53 – 1756.51 μg/gdwt) contents of the samples, however, it decreased the % trans–β-carotene contents. Storage conditions in the study increased Original Research Article Okpalanma; EJNFS, 12(2): 22-39, 2020; Article no.EJNFS.55167 23 significantly (P< 0.05) the contents of total β -carotene and total–carotene except in the case of Gnetum africanum leaf. A regression model for the two methods of analysis of β -carotene with a coefficient of correlation r = 0.925 and coefficient of determination r 2 = 0.856, which allows for the calculation of total β carotene from total-carotene content was obtained.

european journal of nutrition and food safety

Correlation of High Performance Liquid Chromatography (HPLC) and Spectrophotometric Methods to Assess the Post Harvest Storage and Processing Changes in Total β-carotene Contents in Selected Nigeria Vegetables