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Aims: To investigate the isolated flavonoid compound, characterization and antimicrobial activity of Quercetin-3-O-(2”-α-methyl-p-Coumaryl)-rutinoside from Delonix elata flowers. Place and Duration of Study: The research work was carried out at Research laboratory, Department of chemistry, Periyar E.V.R College, Trichy-23, between May 2016 to January 2018. Methodology: Extraction and fractionation was carried out from the solvents of ethanol, benzene, petroleum ether, diethyl ether and ethyl acetate. The structure of the isolated compound (Quercetin-3-O-(2”-α-methyl-p-Coumaryl)-rutinoside) was elucidated through their physical and chemical methods. The isolated compound was characterized by using various spectral data such Original Research Article Rajeshkanna et al.; EJMP, 31(4): 1-11, 2020; Article no.EJMP.54646 2 as UV, 1 H NMR, 13 C NMR, MS. Four bacterial strains Salmonella typhi, Escherichia coli, Enterococcus faecalis, Bacillus cereus and two fungal strains Curvularia lunata and Candida albicans were tested by using disc diffusion method. Conclusion: The present study was concluded that the dry sample of ethyl acetate fraction of Delonix elata flowers was Quercetin-3-O-(2”-α-methyl-p-Coumaryl)-rutinoside and it possesses effective antimicrobial activity against bacteria and fungi.

Isolation, Characterization and Antimicrobial Activity of Quercetin-3-O-(2”-α-Methyl-p-Coumaryl)-Rutinoside from Delonix elata Flowers