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Aims: To determine the effect of the addition of sweet orange essential oil to the extender (tris yolk extender without the addition of antibiotics/antibacterial) to total bacteria Boer Buck frozen semen. Study Design: Randomized Block Design. Place and Duration of Study: Sample: Laboratory Reproduction of Loka Penelitian Kambing Potong Sei Putih Indonesia, between January and March 2019. Methodology: The research procedure starts with the preparation of semen extender, collection of fresh semen, dilution of semen, equilibration, freezing of semen, and thawing. This research obtained conducted using a Randomized Block Design consisting of 5 treatment levels and five replications. Semen storage using 3 Boer buck, which done for three days. As a treatment is the addition of sweet orange essential oil as much as (P0) 0%, (P1) 0,25%, (P2) 0,5%, (P3) 0,75% and (P4) 1% on the tris yolk extender. The observed variables was total bacteria evaluated before freezing and after freezing (Post-Thawing). Original Research Article Sitepu et al.; AJBGMB, 5(3): 1-5, 2020; Article no.AJBGMB.61162 2 Results: The results showed that the addition of sweet orange essential oil had a very significant effect (P <0.01). The results of adding sweet orange essential oil to the extender (Post-Thawing) were 78.6 x 102 CFU (P0), 76 x 102 CFU (P1), 73,2 x 102 CFU (P2), 71 x 102 CFU (P3) and 68.6 x 102 CFU (P4). Conclusion: The best values the total bacteria 68.6 x 102 CFU (P4). It can conclude that the best result of adding sweet orange essential oil to tris yolk extender is (P4) 1%.

Total Bacteria of Post-Thawing Boer Buck Semen with Addition of Sweet Orange Essential Oil to Tris Yolk Extender