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Aims: To assess the effectiveness of triple bagging systems with or without Lippia multiflora leaves on the vitamin-quality of cowpea seeds during storage. Methodology: Cowpea seeds that have undergone any treatment were collected from producer in the southwest of Côte d’Ivoire. The fresh leaves of Lippia multiflora were collected and dried in sunlight for 7 days in center of Côte d’Ivoire. The storage bags used were obtained from the Original Research Article Fofana et al.; AFSJ, 12(4): 1-16, 2019; Article no.AFSJ.51948 2 suppliers to Côte d’Ivoire in Abidjan. All this material was sent to the Laboratory of Biochemistry and Food Sciences, Felix Houphouet-Boigny University, Côte d’Ivoire to perform the experiment. Thus for the experiment realization, a central composite design (CCD) was used. The first factor of CCD consisted to six types of packaging, namely: one control with polypropylene bag (TST), one triple bagging batch (composed of 2 internal layers in independent high density polyethylene 80 mm thick and a woven bag polypropylene) without biopesticide (H0), and four batches (H1, H2, H3 and H4) containing respectively (0.7%; 2.5; 4.3%; and 5%) biopesticide. The second factor, storage time, it included six periods of observation (0; 1; 2; 4.5; 7 and 8 months). HPLC techniques were used for the separation and quantification of β-carotene, α-tocopherol (vitamin E) and the water-soluble vitamins (thiamine, riboflavin, niacin, pyridoxine and folic acid). The estimated daily intake of folic acid (vitamin B9) was also evaluated for Ivorian adult of 70 kg. Results: The results of vitamin properties indicate a significant influence (P < 0.05) between the type of treatments and shelf life. Multivariate analysis results (PCA and AHC) indicate that the addition of at least 0.7% Lippia multiflora leaves in triple bagging systems makes preservation more efficient and preserves the vitamin quality of the cowpea seeds during 8 months. The contribution to meeting vitamin B9 requirements is palpable when cowpea seeds are stored for up to 8 months in the triple bagging systems associated with the biopesticide. Conclusion: Cowpea seeds storage in triple bagging systems with Lippia multiflora leaves appears as a method of effective and inexpensive conservation to ensure the vitamin-quality of cowpea.

Efficiency of Triple Bagging System and Lippia multiflora Leaves for the Vitamin Quality Preservation of Cowpea Seeds (Vigna unguiculata L. Walp)