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Graphene is a monolayer of carbon atoms arranged in a honeycomb lattice and is a basic building block for graphitic materials of all other dimensionalities. It can be wrapped up into zero-dimensional fullerenes, rolled into one-dimensional nanotubes or stacked into three-dimensional graphite. Graphene has unusual electrical, mechanical, and thermal properties, so it presents new opportunities in fundamental research and practical applications (Zhang et al., 2005; Geim & Novoselov, 2007; Geim, 2009). Recently, large-area synthesis methods for graphene have been advanced epitaxial growth on SiC (Brar et al., 2007; Qi et al., 2010) and chemical vapor deposition on metal substrates such as Ni (Dedkov et al., 2008) and Cu (Li et al., 2009). Particularly, Cu is a considerably attractive substrate because it can be produce layer-area graphene. Transmission electron microscopy (TEM) is a direct and re latively fast imaging tool ideally suited for suspended atomically thin membranes, so it has been successfully applied to study adsorbates on graphene and the atomic structure of graphene (Huang et al., 2011). To observe graphene by TEM, the graphene must transfer from metallic Cu substrates to a TEM grid. Standard transfer of layer graphene has been carried out using a polymer coating such as polymethyl methacrylate (PMMA) or polydimethylsiloxane as a temporary support during etching of the metal substrate to prevent tearing of the graphene (Reina et al., 2009). However, the standard transfer method using these polymers can contaminate and mechanically damage the graphene because it includes several wet chemical steps. Thus, a simple and gentle method is necessary for good TEM images of graphene. Direct transfer method doesn’t include polymer coating process. So it is expected simple and effective method to make graphene specimen by avoiding wet chemical steps (Regan, 2010).

Transmission Electron Microscopy Specimen Preparation for Layer-area Graphene by a Direct Transfer Method