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Department of Food Science and Engineering, Seowon UniversityAbstract To analyze the characteristics of bacteriophages in Enterococcus faecium, D-19 and F6 phages were inducedfrom five E. faecium isolated from sprouts by the treatment with mitomycin C. The bacteriophages of D-19 and F-6 hadlong, non-contractile tails and icosahedral heads, and were members of Siphoviridae family. As the host spectrum, D-19phage lysed five out of 55 strains of E. faecium, whereas F6 phage lysed only three strains. Both D-19 and F6 phagesdisplayed similar and high stabilities against ethanol and pH capable of resisting the exposure to 100% ethanol and pH 4.Keywords: Enterococcus faecium , temperate phage, Siphoviridae, host spectrum, stability

Isolation and Characterization of Temperate Phages in Enterococcus faecium from Sprouts