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Hee Young Jeon, Seung Hun Kim, Hyun Jeong Shin, Dae Bang Seo, and Sang Jun Lee*Amorepacific Corporation R&D CenterAbstract Currently, various treatments available for alleviating hair loss and combination treatments are commonly used,which are frequently questionable and in effective. We aimed to investigate the synergistic effect regarding the combinationof oral herbal composition and topical hair tonic on anagen induction and hair restoration in a shaving model of C57BL/6 mice. Seven-week old mice were trimmed by electric clippers and treated with oral herbal composition and topical hairtonic either alone or in combination. The combination treatment showed the highest hair growth promoting effect.Moreover, it significantly improved total blood antioxidant capacity and reduced lipid peroxidation and triglyceride level,which was not observed in the topical hair tonic treated group. These results suggest that the combination of oral herbalcomposition and topical hair tonic has a synergistic hair growth promoting effect and such synergism may be the resultof the differing hair regrowth mechanisms concerning treatments.Keywords: hair loss, oral and topical, combination, synergy, C57BL/6 mice

Synergistic Effects of the Combination of Oral Herbal Composition and Topical Hair Tonic on Hair Growth in C57BL6 Mice