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Department of Food Science and Technology, Pukyong National UniversityAbstract Zearalenone (ZEA) is an estrogenic mycotoxin mainly produced by Fusarium graminerum, a species whichcolonizes a wide variety of cereals, including wheat, barley and processed products. A survey of ZEA contamination wasconducted on 141 dried confectioneries, 59 breads and rice cakes, 135 noodles and 101 other products, for a total of 432commercial samples. Samples were analyzed by high performance liquid chromatography with fluorescence detection(HPLC-FLD) after immunoaffinity clean-up and was confirmed by liquid chromatography tandem mass spectrometry (LC-MS/MS). The limits of detection and quantification were 2.0 and 6.0µg/kg, respectively. The recovery ranged from 80.2%to 98.4% in the cereal based product. ZEA was detected in 38 samples (8.8% incidence), including 3 snack, 2 biscuit and33 other cereal products. The ZEA contamination levels were in the range of 5.38-53.76 µg/kg. Finally, LC-MS/MSanalysis of the contaminated samples was conducted to confirm the detected ZEA, and all 38 samples showing ZEA byHPLC-FLD were confirmed by LC-MS/MS.Keywords: mycotoxin, zearalenone, immunoaffinity column, cerealFusarium             Bakanae     ,  , ,     (1).   Fusarium        zearalenone,deoxynivalenol, fumonisin         (2-4).          ,          ,      .    ·           (5).     Fusarium graminearum, Fusarium moniliforme     , ,          (6). (International Agencyfor Research on Cancer, IARC)       group 3        , ,    , ,         (7-9). Lee (10)                         .                 ,   estradiol   estrogen     .      polychrorinated biphenol(PCB) bisphenol A .         20 mg/kg- 200 mg/kg  JECFA(The Joint FAO/WHO Export Committee on Food Addi-tives)     (provisional maximumtolerable daily intake, PMTDI) 0.5 mg/kg body weight/day,(EU Scientific committee for food)0.2 mg/kg body weight/day (11,12).       , ,    . 1999 Placinta*Corresponding author: Mi-Ran Jang, Hazard Substances AnalysisTeam, Test & Analytical Center, Busan Regional KFDA, Busan 608-829, KoreaTel: 82-51-610-6181Fax: 82-51-610-6199E-mail: jang9797@hanmail.netReceived January 5, 2010; revised March 18, 2010;accepted December 12, 2010

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Analysis of Zearalenone Contamination in Cereal-Based Products Using High Performance Liquid Chromatography-Fluorescence Detector and Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry