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Hyaluronic acid(HA) microbeads were synthesized by dropping the sodium hyaluronate(Streptococcus)solutions in NaOH into a solution mixture of divinyl sulfone(DVS) in 2-methyl-1-propanol, followed by stirring, clean-ing and drying process at room temperature. The initial experimental conditions are crosslinking time(CLTi) of 5 h,crosslinking temperature(CLTe) of room temperature, injection air pressure(IAPr) of 5 psi, and DVS concentra-tion(DVSc) of 0.2 vol%, respectively. Then, parametric studies were performed by varying the parameters to inves-tigate the morphology, the porosity, the swelling ratio and the size of the beads. The microbead size pattern was notregular to function of the degree of crosslink. It was observed that the swelling ratio, the degree of crosslink, andthe pore size can be controlled by adjusting the CLTi, CLTe and DVSc. Among the parameters investigated, the small-est bead size can be achieved by varying the CLTi parameter. The lowest swelling ratio, as an indication of the highestdegree of crosslink, can be obtained by varying CLTe.Key words: Hyaluronic acid microbead, Tissue regeneration, Hydrogel

Characterization of Cross Linked Hyaluronic Acid Microbeads by Divinyl Sulfone