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This paper presents a method for the rapid detection of crude oil in the presence of large amounts of biological material. The method involves the use of high pressure liquid chromatography with chloroform as the solvent, an excitation wavelength of 403 nm, and an emission wavelength in the range of 418 nm. Of the five crude oils employed in this investigation, Saudi Arabian crude oil was the least responsive to the above technique and yet is still detectable in quantities of less than 1 µg. Reproducibility studies indicated that the percent error of repetitive injections of Saudi Arabian crude oil samples was 1.3%. Repetitive injections of spiked shrimp samples gave a percent error of 3.7%. This is excellent reproducibility for residue-type samples which undergo a number of manipulations during workup. Thus, this method is selective, sensitive, reproducible, and affords a rapid analysis.

A LIQUID CHROMATOGRAPHIC FLUORESCENCE TECHNIQUE FOR ESTIMATING CRUDE OIL IN WATER, SEDIMENT, AND BIOLOGICAL MATERIAL