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The study is focused to biosynthesize silver nanoparticles using lemon peel extract and to assess the antibacterial activity of AgNPs against the selected urinary tract pathogens. 4 g of finely cut Citrus limon peel was added to 40 ml double distilled water, boiled for 2 minutes and filtered. 3 ml of the extract was added to 40 ml of 1 mM AgN O 3 solution and change in color was observed. The bio reduction of Ag + ion in solution was monitored using UV – visible spectrometer, FESEM and EDAX analysis. FESEM analysis showed the presence of AgNPs with the size between 17.3 - 61.2 nm and the shapes were spherical and some were irregular. The energy dispersive X - ray analysis (EDAX) revealed strong signal at 3 keV, in the silver region and confirmed the formation of silver nanoparticles. The extract was tested against the urinary tract pathogens like Pseudo monas aeruginosa, Escherichia coli, and Klebsiella pneumonia. The silver nanoparticles showed maximum zone of inhibition of 24 mm ± 0.3SD, 14 mm ± 0.2 SD, 12 ± 0.5 SD against Pseudomonas aeruginosa, Escherichia coli, and Klebsiella pneumonia respectively. The present research work emphasizes the use of lemon peels for the effective synthesize of AgNPs and could be used against the urinary tract pathogens which are found to develop drug resistance towards broad - spectrum antibiotics.

journal of biological and scientific opinion

BIOSYNTHESIS OF SILVER NANOPARTICLES USING CITRUS LIMON (LINN.) BURM. F. PEEL EXTRACT AND ITS ANTIBACTERIAL PROPERTY AGAINST SELECTED URINARY TRACT PATHOGENS