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Areca catechu L. nut is a component of betel quid, the chewing of which is a popular or a cultural tradition in some countries in South East A sia. Areca nut contains bioactive components like alk aloids and tannins which have been demonstra ted to elicit inhibitory effects in selected microorganisms. Varying concentrations of Areca catechu L. nut ethanol extract were tested for antimicrobial activity against 0.5 McFarland of mixed - oral flora and eight gram - negative clinical isolates ( E. coli, K. pneumonia, P. vulgaris, P. aeruginosa, S. non - t yphi, S. typhi, S. flexneri and V. c holera ) by agar well diffusion method. All concentrations were shown to inhibit growth in all mixed - oral flora models with zones of inhibition ranging from 7 mm to 18 mm . Susceptible patterns were also seen in all gram - negat ive clinical isolates with the smallest mean of zones of inhibition seen in Escherichia coli which is 8 mm at 30 % concentration and Klebsiella pneumoniae with no zone of inhibition in b oth 30 % and 50 % concentration. Highest antimicrobial activities were seen against Proteus vulgaris and Vibrio cholerae with a mean zone of inhibition of 18 mm at 70 % concentration and a mean zone of inhibition of 16 mm at 50 % concentration respectively. T h ese results we re comparable to intermediate susceptible pattern in Ciprofloxac in which is 16 mm to 20 mm and indicates that there are bioactive components present in areca nut that are worthy enough of further study and exploitation for its inherent antimicrobial act ivity.

ANTIMICROBIAL PERFORMANCE OF ETHANOLIC EXTRACT OF ARECA CATECHU L SEEDS AGAINST MIXED-ORAL FLORA FROM TOOTH SCUM AND GRAM NEGATIVE LABORATORY ISOLATES