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physical properties of culture medium, and moreover, on the growth of various microorganisms in an organ of living animal. Recently, HORIUCHI 1) and HORIUCHI u. OKABE 2) reported that a testicle of hog contained a substance which effected a good growth of Bac. pertussis and Bac. fusiformis specifically. HIWATASHI 3) reported a study of various infusions of organs in the culture of Bac. turalensae and pointed out that an infusion of hog's liver contained a substance which effected a good growth of this organism, and he thought that it was due to the cystine or the like substance in it. He also examined the blood of some animals and said that cystine seemed to be used not only as a growth promoting factor but also subsequently as a nutrition for the organisms, and that the horse blood seemed to be lacking in growth promoting factors. FUJIWARA 4), on the other hand, made studies of shake tube culture of various microorganisms and found that Streptococcus haeynolytieus, Streptococcus viridans, Staphylococcus, and Diplococcus pneumoniae grew microaerobically in glucose agar and that they grew in the whole medium, however, in simple agar medium, he pointed out that we might approach the optimal conditions of growth of microorganisms in the initial stage of growth which could not be understood only in view of nutrition. It seems that only a few reports have been made on the shake tube culture of Bac. pestis in glucose agar medium since ALBRECHT u. GOHN 8) reported that it grew in the whole tube of the medium. Recently, detailed studies and reports were made on this subject by S. AMANO 5). In the present study which was made following AMANO's experiments, various infusions of animal organs were used instead of the meat extract •g Lender•h which was used in the said experiments. As the author believes that the liver infusion was found to give special effects for the growth of Bac. pest. and Bac. pseudotbc., the results obtained from those experiments are reported in the following.

ON CULTIVATION OF BAC. PESTIS IN THE TUBE OF GLUCOSE AGAR CONTAINING THE INFUSION OF ANIMAL ORGAN