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proving, but in this study 30% formalin was also used for fixing. After the staining by purplin, the tissues were washed with distilled water 2 minutes, differentiated with 70% alcohol, then enclosed by glycelin. For a control, the specimen treated with 1/10N-chloric acid 2 minutes was used to compare. By these observations, calcium was not proved in eggs and surrounding tissues throughout every stage of ascaris oð±genesis. In order to prove the calcium

japanese journal of medical science and biology/japanese journal of medical science and biology

BIOLOGICAL STUDIES ON ASCARIS EGGS V. ON THE STAINING REACTIONS OF ASCARIS EGGS TO PHOSPHOR, CALCIUM AND SOME OTHER SUBSTANCES IN THE PROCESS OF THEIR O^|^Ouml;GENESIS