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Behre and Benedict(1) developed a procedure for the determination of ergothioneine by its reaction with a phosphotungstic acid reagent, and Huntert(2) described a method based upon the reaction between the diazotized sulf anilic acid and ergothioneine. But these procedures seemed to be unsatisfactory, so in 1947 we devised a new methodt(3) based upon the conversion of the sulfur of ergothioneine to the sulf ate by the alkaline perhydrol (Kitamura's reaction)(4) and estimated the quantity of ergothioneine in urine, blood and organs of animals and of several patients.

ESTIMATION OF ERGOTHIONEINE IN URINE, BLOOD AND ORGANS BY DETERMINATION OF THE ALKALINE PERHYDROL-LABILE SULFUR