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The 7S and 19S fraction isolated from hyperimmunized antileptospiral horse sera by Sephadex G-200 gel filtration and gamma- and T-globulin fractions from the antisera by starch block electrophoresis were studied for neutralizing (leptospiricidal) activities against infection with Leptospira. The activity was determined by the intracutaneous method with virulent Shibaura Strain of Leptospira interrogans serovar copenhageni. No significant difference in regression coefficient was observed between the neutralization line with the 19S globulin fraction and that with the 7S globulin fraction, whereas a significant difference in the regression coefficient was observed when the gamma- and T-globulin fractions were compared; the regression coefficient with T-globulin was consistently larger than that with gamma-globulin.

ANALYSES FOR NEUTRALIZING ACTIVITIES OF IMMUNOGLOBULIN FRACTIONS IN ANTI-LEPTOSPIRAL HORSE ANTISERUM