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Precise and reproducible quantitative determination of hepatitis B surface antigen by applying the parallel line bioassay method was proposed. In this method, the value of any given sample can be expressed in terms of a relative value to a fixed standard or reference preparation. Decrease in antigenic reactivity due to the lowered protein content could be prevented by using 0.02% human albumin in physiological saline solution as the diluent. The use of such stabilizer(s) should be considered in the dilution or storage of samples containing HBs antigen.

APPLICATION OF THE PARALLEL LINE BIOASSAY METHOD TO QUANTITATIVE DETERMINATION OF HBs ANTIGEN IN RADIOIMMUNOASSAY