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In vitro production of enterotoxin by Vibrio cholerae, NAG isolated from human diarrheal cases in India, the Philippines and Sudan was tested. Of a total of 41 strains tested, 8 proved to produce the toxin characterized by permeability-increasing activity in the guinea pig skin. Characterization of the toxin produced by a representative strain was also made in comparison with cholera enterotoxin produced by V. cholerae, serotype 1, 569B strain. This toxin was heat labile, nondialyzable and immunogenic. It evoked

IN VITRO PRODUCTION OF ENTEROTOXIN AND HEMORRHAGIC PRINCIPLE BY VIBRIO CHOLERAE, NAG