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The acetone-ether extracted Japanese encephaltis virus (JEV) hemagglutinin (HAnin)** from infected suckling mouse brains, originally containing about 108.0 infective virus particles per ml, was mixed with a goose red cell suspension with a concentration of 3 •~ 108.0 cells per ml at an iced bath temperature. Adsorption of HAnin onto goose cells took place in a narrow range of pH, generally between 6.4 to 7.0; no adsorption occurred at pH 9.0 within 30 minutes after mixing. The adsorption of HAnin onto goose cells is definitely pH dependent. The optimum pH for adsorption coinsides with that for the pattern hemagglutination (HA) test.

ADSORPTION OF JAPANESE ENCEPHALITIS VIRUS HEMAGGLUTININ ONTO GOOSE RED CELLS