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The materials and methods are similar as used in our previous paper (Kato et al. 1963), unless otherwise described. Two kinds of media differing from each other only in the iron concentration were used . One of the media was added with 0.01ƒÊg of ferrous ion per cc to make the concentration of iron optimal for the toxin synthesis, and the other with 4.0ƒÊg per cc to give an excess of iron . The total quantity of diphtheria toxin protein synthesized at the time of harvest of cells were about 160ƒÊg per cc with the medium without excess of iron and about 5ƒÊg per cc with the medium with excess of iron. The ribosome and the supernatant fractions obtained from cells

REGULATORY MECHANISM OF THE SYNTHESIS OF DIPHTHERIA TOXIN IN THE CELL-FREE SYSTEM INHIBITORY FACTORS IN CELLS GROWN IN THE MEDIUM WITH EXCESS OF IRON