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Waksman and his collaborators (1) found that streptomycin-producing strains were much more resistant to streptomycin than other strains of streptomyces, and the isolation of streptomycin-producing strains became much easier. After a chloromycetin-producing strain was isolated by us (2), the further isolation of such a strain has been very difficult. During testing resistances of antibiotic streptomyces to chloromycetin, we found that the chloromycetin-producing strain was more resistant to chloromycetin than other strains. So in further trials of isolating chloromycetin-producing strains, the soil was streaked on the nutrient agar added with chloromycetin. Then from colonies growing on the agar chloromycetinproducing strains were easily obtained.

RESISTANCES OF ANTIBIOTIC STRAINS OF STREPTOMYCES TO CHLOROMYCETIN AND A RAPID ISOLATION METHOD OF CHLOROMYCETIN PRODUCING STRAINS