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The pre-spore cells in D. discoideum were focused upon as they produce extracellular signals (cAMP and DIF-1) involved in cell differentiation. To further investigate the role of filamin, the filamin promoter gene was firstly amplified by PCR. This was then ligated into a plasmid containing a lacZ reporter gene using the restriction enzymes Bam and Eco . This was next transformed into E. coli for further growth and subsequently electroporated into Dictyostelium cells. Following a period of growth under optimum conditions, the cells were stained using X-Gal under the presence of a pH 7.0 Z-buffer, allowing areas of high expression to be identified. To gain insight into its influence upon cell sorting, UblA knockouts were created and the results were recorded.

clinical medicine

The study of cytoskeletal regulators on cell motility using Dictyostelium discoideum as a model organism with a view to human application in Marfan's syndrome and valvular diseases