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Toxicity and uptake of 2,4,6-trinitrotoluene (TNT) in three different soils (OECD soil, natural soil and loess) to earthworm(Eisenia fetida) were investigated at several different spiked concentrations of TNT (0 to 200 mg/kg for OECD and naturalsoils, and 0 to 35 mg/kg for loess) and for different exposure periods (7, 14, 21, and 28 d). The LC 50 values for 7 dexposure were 160.1, 159.4, and 28.81 mg/kg for OECD soil, natural soil, and loess, respectively. The LC 50 values for 14,21, and 28 d exposure were almost the same as those for 7 d exposure, showing that 7 d exposure time was enough todecide the toxicity (LC 50 ) of TNT to Eisenia fetida, because the highest concentration of TNT in earthworm body wasobserved within around 5 d. The LC 50 and uptake of TNT in loess were higher than those in OECD and natural soil. Theuptake of TNT to the earthworm were correlated well with the initial concentration of TNT in the soil and TNT porewaterconcentration (R 2 > 0.9 in OECD, natural, and loess). The concentration of TNT in earthworm body decreased after 5 d,possibly caused by natural degradation of TNT by soil bacteria as well as earthworm.Key words : 2,4,6-trinitrotoluene, Eisenia fetida, Soil, Toxicity, Uptake

Toxicity and Uptake of 2,4,6-Trinitrotoluene (TNT) in Contaminated Soils to Eisenia fetida