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A surface plasmon resonance imaging (SPRI) assay was developed for measuring porcine circovirus type 2 (PCV2) antibody using a recombinant capsid protein as an antigen. The diagnostic potential of SPRI for detecting antibodies to the PCV2 capsid protein was compared with that of a conventional enzyme-linked immunosorbent assay (ELISA) using 70 pig serum samples taken from 6 pig farms. There was a strong positive correlation between the SPRI and ELISA (n = 70, r = 0.911, P 0.01). Therefore, this recombinant capsid protein can be used as an antigen for serological studies, and the SPRI, a label-free and high-throughput method, is expected to be a valuable tool in the serodiagnosis of PCV2 infection. Key words : Porcine circovirus type 2, Surface plasmon resonance imaging, Protein chipINTRODUCTION Porcine circovirus type 2 (PCV2) is the primary causa-tive agent of several syndromes collectively known as porcine circovirus-associated disease (PCVAD) (Gillespie et al, 2009). PCVAD is a globally emerging disease that is having a huge impact on swine-producing countries and is arguably the most economically important disease affecting the global swine industry today (Gillespie et al, 2009; Opriessnig et al, 2007). PCV2 is a small, non-enveloped, circular DNA virus that is only known to cause disease in pigs. The PCV2 has two major open reading frames (ORFs). ORF1 is es-sential for viral replication, while ORF2 encodes a ma-jor capsid protein (Nawagitgul et al, 2000). The ORF2 protein contains the type-specific epitopes (Mahe et al, 2000), which suggests that ORF2 contributes to the de-velopment of the disease, the vaccine potential (Liu et al, 2000), and type-specific diagnostic potential(Blanchard et al, 2003). Epidemiological data suggest that the virulence of the disease is strongly related to the presence of the capsid protein (Nawagitgul et al, 2000). Several diagnostic methods for detecting PCV2 anti-bodies of pigs have been developed, which include neu-tralizing antibody test and enzyme-linked immuno-sorbent assay (ELISA) (Cho et al, 2006; Meerts et al, 2006). Although these methods are quite reliable, they are time- and labor-intensive protocols. Most protein ar-rays currently developed rely on detection technologies that employ enzymatic or fluorescent tags. In contrast, surface plasmon resonance (SPR) as a label-free technol-ogy for monitoring biomolecular interactions is a prom-ising technique for rapid and parallel detection (Cho et al, 2006). SPR imaging (SPRI) using fast optical array detectors permits simultaneous measurements across an array of immobilized molecules. SPRI provides excellent spatial resolution at the same sensitivity as classical

Development of serodiagnostic surface plasmon resonance imaging assay for the detection of antibodies to porcine circovirus type 2