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This study was aimed for development of a useful genes that has a transcript expressional specificity in the early embry-onic stage of the silkworm, Bombyx mori. We constructed and analyzed a full-length cDNA library from silkworm ’s eggs which after a lapse of 2~ 6 hours post oviposit. A total 960 clones were randomly selected, and the 5’ ends ofthe inserts were sequenced to generate 652 expressed sequence tags(EST). 334 unique ESTs were generated after theassembly of 652 ESTs. The annotation of 334 unique ESTs by BLAST search revealed that 156(47%) of the sequencesrepresented known genes, whereas 178(53%) of the sequences has no matches in the database. Of the 156 known genes,the most abundant genes were heat shock protein hsp20.8 gene(12 times) and ubiqutin-like protein gene(11 times). Thefunctional groups of these ESTs with matches in the database were constructed according to their putative molecularfunctions. Among thirteen functional categories, the largest groups were protein synthesis(9.6%) and cellular orga-nization(8.1%). Further defined studies on molecular functions and biological roles of their promoters will give us well-fined information and its application. Key words : Silkworm, Embryo, cDNA library, EST

Gene expression profile of the early embryonic gene of the silkworm, Bombyx mori