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The antifungal efficacy of tangerine oil at various concentrations (10-200 μL/mL) were tested in agar medium and on rubberwood surface against Aspergillus niger indentified from raw and boron treated rubberwood. Various dilutions were made in methanol and vegetable oil was used as a control. Components of the tangerine oil were determined by means of gas chromatography-mass spectrometry (GC–MS) analysis. Minimum inhibitory concentrations (MICs) of tangerine oil against A. niger identified from raw and boron treated rubberwood in agar medium were 50 μL/mL and 180 μL/mL, respectively. In addition, tangerine oil at those concentrations were capable of protecting against mold growth of A. niger on rubberwood surface for at least 12 weeks under storage condition at 25ºC and 100%RH. Major constituents of tangerine oil identified were limonene (69.2%), geranial (16.4%), and p-cymene (4.7%). Higher resistance to tangerine oil of A. niger identified from rubberwood treated with boron indicates that boron might enhance mold resistance of A. niger to essential oil

Effect of Tangerine Oil against Aspergillus niger Identified from Raw and Boron Treated Rubberwood