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The increasing demands of consumers for using less chemical preservatives has led to increasing number of researches to produce natural compounds with antimicrobial properties. Fresh aerial parts of wild Ziziphora tenuior were collected in Eastern Iran. Z.tenuior samples were collected after initial preparation isolated from the aboveground plant parts and plant samples preparation by water distillation using Clevenger apparatus after determining the optimal conditions for making essential oil was obtained. Essential oil with an efficiency of 1/1 percent (g/g) was obtained and by GC-MS to determine the composition of the essential oils was analyzed and indicates 21 compounds. Key combinations were as: pulegone (82.6 %) and limonene (6.8%) which was extracted. the studied essential oil would exhibit good antimicrobial properties, this essential oil in 0.08 ml concentration could eliminate microorganisms in water. but this was not evaluated and further investigation should be carried out for use of this essential oil in various industries.

Antimicrobial Effect of Essential Oil of Ziziphora tenuior on Water by Heterotrophic Plate Counts Method in Kerman (Southeast of Iran)