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—Mangosteen pericarp has been used for long time as traditional medicine. One of main active ingredient in mangosteen pericarp is xanthone. Xanthone has remarkable effects on cardiovascular, antiviral, and anti-inflammatory. Subcritical ethanol extraction (Sc-ethanol) was employed to extract xanthone from dried mangosteen pericarp and compared with maceration and soxhlet extraction. The Sc-ethanol was applied by various temperature (80, 120 and 160 o C), ethanol concentration (50, 72.5 and 95%) and extraction time (10, 20 and 30 min.) for 1:20 of sample to solvent ratio. The Box-Behnken design was applied to investigate the optimum condition of Sc-ethanol extration. The optimum conditions from Box-Behnken design to obtain the highest xanthone were determined at the optimum conditions as following; temperature 160 o C, extraction time 30 min in 95% ethanol. The results presented that for maceration, soxhlet and Sc-ethanol extractions in 0.5 h, the extracted xanthones were 28.31, 31.26 and 57.42 mg/g of dried mangosteen pericarp respectively.

Optimization of Subcritical Ethanol Extraction for Xanthone from Mangosteen Pericarp