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Anthracnose is the most important fungal disease on Persian walnut in Iran. The current study was performed on 16 Ophiognomonia leptostyla isolates collected from northwest region of Iran. ITS amplification was performed using ITS1 and ITS4 primers and the sequences were analyzed. Phylogenetic trees made based on ITS sequences showed that all Iranian isolates are in the same branch in a clade with Ophiognomonia leptostyla from CBS isolated from Juglans regia. The Iranian isolates are divided into two closely related sub-clades with low bootstrap support, the smaller sub-clade comprised of only three isolates were collected from wild walnut trees located in woodland areas. Keywords-Gnomonia leptostyla; walnut leaf blotch; phylogentic tree; ITS sequencing I. I NTRODUCTION

Molecular Phylogeny of Ophiognominialeptostyla Isolates Collected from Iran Based on ITS nrDNA Sequences