English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

: Cowper chlorotic mottle virus (CCMV) is the ‘controlled’ quarantine virus as plant pathogenic virus that are classed as group Ⅵ (+) ssRNA virus that belongs to the genus Bromovirus and family Bromoviridae , When plants that are Phaseolus vulgaris , Clitoria ternatea , Nicotiana tabaccum , Glycine max , Vigna unguiculata and Vigna siensis , and Arachis hypogaea is imported in domestic. In this study, inspection system is implemented to analyze CCMV accurately and rapidly by developing RT-PCR, nested PCR, and gene insertion positive control. It is expected that the method developed in this study will contribute to the plant quarantine to be consistently utilized in the field. Key words : Cowpea chlorotic mottle virus , CCMV, Nested PCR, Quarantine *Corresponding author: Tel: +82-32-560-8354E-mail address: siwonlee@korea.kr I.   Cowpea chlorotic mottle virus (CCMV) Group IV positive sense single strand RNA virus, Bromoviridae, Bromovirus   , Bancroft (1967)   . CCMV  30 nm  20   3 RNA   .   , CCMV  RNA1 (3.17 Kb), RNA2 (2.77, Kb)  RNA3 (2.17 Kb) , RNA1 1a protein, RNA2 2a protein  RNA3 movement protein coat protein  . , CCMV ( Phaseolus vulgaris), (Clitoria ternatea), (Nicotiana tabaccum), (Glycine max), (Vigna unguiculata, Vigna siensis)  (Arachis hypogaea)   ,    (Animal, Plant and Fisheries Quarantine and Inspection Agency, 2013; Lee et al., 2013c). 2006 CCMV enzyme-linked immunosorbent assay (ELISA)  (Stein et al., 1979),     (Caruso et al., 2003; Priou et al., 2006)      (Lee, 2013).  RT-PCR            (Kim et al., 2000, 2005; Park and Kim, 2004; Lee et al., 2011a; Lee et al., 2011b),    RT-PCR   , nested PCR   (Lee et al., 2013a; Lee et al., 2013b; Lee et al., 2013c). ,         PCR

Development and of Diagnostic System for Detection of Cowpea chlorotic mottle virus using by Nested PCR