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- This study was conducted to establish the in vitro optimal condition for seed germination and organogenesis of wild Angelica gigas . The experiment was evaluated the effects of GA 3 for pre-treatment with different periods of time (0h, 24h, 48h, 72h) and followed the treatment of seeds by control, scarification and methanol-heating method. As a result, the highest rates (15%) of seed germination was shown under the treatment without soaking of GA 3 and methanol-heating treatment. The seed germination was highly increased 60% unde r the condition of treatment on ultrasonic waves (frequency 80 KHz) with methanol-heating treatment including 0.1 mg/L GA 3 . The highest callus induction rate was obtained from in vitro germinated stem, root and hypocotyl on the MS medium with 1.0 mg/L NAA and 0.5 mg/L BA. The highest percentages of shooting (50%) and rooting (85%) induction were observed in hypocotyl and root cultured on PGRs free medium and 0.1 mg/L NAA, respectively. In addition, somatic embryogenesis was observed from stem (1.0 mg/L 2,4-D) and hypocotyl (0.1 mg/L NAA).

Effects of Plant Growth Regulators on In Vitro Germination and Organ Formation of Wild Angelica gigas N.